翻訳と辞書 Words near each other ・ Cell Therapy ・ Cell therapy ・ Cell tower technician ・ Cell transfer therapy ・ Cell Transmission Model ・ Cell type ・ Cell Walk for Celeste ・ Cell wall ・ CELL セル ・ Cell-based vaccine ・ Cell-cycle analysis ・ Cell-cycle nonspecific antineoplastic agents ・ Cell-free fetal DNA ・ Cell-free marginal layer model ・ Cell-free protein array ・ Cell-free protein synthesis ・ Cell-free system ・ Cell-free tumour DNA ・ Cell-mediated immunity ・ Cell-penetrating peptide ・ Cell-probe model ・ Cell-Scape ・ Cella ・ Cella (disambiguation) ・ Cella (Yup'ik word) ・ Cella Dati ・ Cella De Loanga ・ Cella Delavrancea ・ Cella Dwellas ・ Cella Monte Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Cell-free protein synthesis ： ウィキペディア英語版 Cell-free protein synthesis Cell-free protein synthesis (also called in-vitro protein synthesis or abbreviated CFPS), is the production of protein using biological machinery without the use of living cells. The in-vitro protein synthesis environment is not constrained by a cell wall or homeostasis conditions necessary to maintain cell viability. Thus CFPS enables direct access and control of the translation environment which is advantageous for a number of applications including optimization of protein production, optimization of protein complexes, to study protein synthesis, incorporating non-natural amino acids, high-throughput screens, and synthetic biology.. ==Introduction== Common components of a cell free reaction include a cell extract, an energy source, a supply of amino acids, cofactors such as magnesium, and the DNA with the desired genes. A cell extract is obtained by lysing the cell of interest and centrifuging out the cell walls, DNA genome, and other debris. The remains are the necessary cell machinery including ribosomes, aminoacyl-tRNA synthetases, translation initiation and elongation factors, nucleases, etc. Two types of DNA can be used in CFPS: plasmids and linear expression templates (LETs). Plasmids are circular, and only made inside cells. LETs can be made much more effectively via PCR (see polymerase chain reaction), which replicates DNA much faster than raising cells in an incubator. While LETs are easier and faster to make, plasmid yields are usually much higher in CFPS. Because of this, much research today is focused on optimizing CFPS LET yields to approach the yields of CFPS with plasmids. An energy source is an important part of a cell-free reaction. Usually,a separate mixture containing the needed energy source, along with a supply of amino acids, is added to the extract for the reaction. Common sources are phosphoenol pyruvate, acetyl phosphate, and creatine phosphate. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Cell-free protein synthesis」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.